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Why pyrene is also use for calibration if detector
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Bcoz. It gives higher absorbance band ,lambda max 373 nm .rather than caffeine. That's why
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In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
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Organic Chemistry 
