How to select HPLC column for particular product.
Answers were Sorted based on User's Feedback
Answer / yemulas(ycs)
the column selection is depends upon the polarity of the sample or drug
if in case the drug is highly polar choose highly non-polar column (c18,c8)
if in case the drug is low polar choose the low non-polar column (cyno,c4)
simple :--
sample polar then take --- non-polar column
sample non-polar then take ---polar column
Is This Answer Correct ? | 26 Yes | 1 No |
Answer / lava khandelwal
if sample is basic then use BDS column & if acidic then use
ODS column.
Is This Answer Correct ? | 27 Yes | 6 No |
Answer / fareena
it depends upon the the properties and nature of analyte
because selection of column means selection of stationary
phase.furthermore it also depends on the type of product
i.e. multiactive(more than one active),needs a better
resolution of peaks by increasing column lengh.
Is This Answer Correct ? | 7 Yes | 4 No |
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