What is the difference between void volume and delay volume
in HPLC?

Answer Posted / priyanka nayani

Delay volume is the volume between GPV & Detector.
Dead volume is the width of pipe that is the connection between column out let and detector(0.1mm or 0.01mm) based on the size which we have introduced the void volume changes.

Is This Answer Correct ?    0 Yes 1 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

iam usig ph buffers merk. manually how to prepare ?

1786


what is third integration

1504


why does used dry caffien in HPLC calibration?

1571


why digestion require in icpms?

309


what is mean by nitrosamine impurities?

546






before starting analytical method valodation what you checking? and how giving preference to start validation?

353


If we have 5 strength which is not dose proportinate and excipients also diffrent in each strength then how we can proceed for Force degradation? and excipient are same but not dose propotinate the how FD?

337


if content uniformity passing but dissolution varrying then what is next step?

394


What is the requirement for brazil in the analytical method vslidation comparr to ich?

278


what is diffrence between specificity and selecivity?

320


What is delay volume?

858


why glutent are detected in the rice cereal baby food product even manufacturer claimed that they are using rice and milk only?we have using ELISA to do the test,and rice supposed not containing any glutent,rite?We already repeat the test so many times and it still detected.just wondering where the glutent came from?

2350


How we can identify process related and degradation impurity in single method with short period?

340


Related substance method equivalency on control sample or spiked sample?

308


How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?

2723