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Why we use toluene for resolution in UV calibration?
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Answer / vasudha
The Spectral Bandwidth (SBW) of a spectrophotometer is the
basis of establishing its ability to resolve absorbtion
lines seperated by small differences in wavelength. If a
substance that you need to measure has an absorbtion line
at 257.4nm and there is an interfering line at 260.0 nm you
need to establish whether your spectrophotometer can
resolve the two lines seperately or "mixes" the two
absorbtion lines into a single line which would give
erroneous results.
Toluene in Hexane is used as a reference for the
calculation of SBW. The ratio of the absorbtion of the
solution when read at a maxima (around 268.7nm) and at a
minima (around 267.0nm) relates directly to the SBW of the
instrument being assessed. Regular use of this technique
will assure that the resolution of your instrument is
within the required range for your work
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