how calibrate the hplc detector
Answers were Sorted based on User's Feedback
Answer / m.sivalingeswara rao
Hplc detector calibration to be done to know the acuracy of
the emitted light wave length to the set value as well as
to check the sensitivity of the detecotr to respond with
the concentration of analyte.
To check accuracy generally caffeine solution is used
because it shows maxima and minima at particular wave
lengths(i.e maxima at 206nm,273nm and Minima at 241nm,in
this range we can check accuracy of lamp emitted light).
Sensitivity by permorming linearity test with different
concetration of caffeine solution.
Reproducibility by injecting the replicate injection of
Caffeine solution and measuring the %RSD of the responses
Is This Answer Correct ? | 19 Yes | 0 No |
Answer / samba siva reddy.p
detector checking with caffiene soln bcz caffiene have 2 max and 1 minima wavelenths,we inject std stock soltn(caffiene soln) with diffrent wavelengths.
201 to 209= 205(maxima)
241 to 249=245(minima)
269 to277=275(maxima)
limits+ or -2
Is This Answer Correct ? | 2 Yes | 0 No |
how will you do the prep for unstable componds?
0 Answers AMRI, CTX Life Sciences, Piramal Healthcare,
how calibrate the hplc detector
2 Answers Cipla, Shraddha Analytical Services,
IN pH CALIBRATION,CALIBRATION IS DONE FOR pH METER OR ELECTRODE?HOW WILL YOU JUDGE THAT THE METER TO BE CALIBRATED WITH 4,7,9.2 BUFFERS?EXPLAIN TEMPERATURE EFFECTS ON pH.
what is GLP? Which industries it requried?
1 Answers Aurobindo, AXIS Clinicals, GVK,
What is the column performance and column efficiency?
what is protocol ? plz Explain me
Why we use Uracil for HPLC calibration in Detector wave ength accuracy test?
We use Potassium Dichromate solution for the calibration of UV-Visible Spectrophotometer in UV region. My question is to calibrate visible region which solution could be used in photometric accuracy
why kf factor coming 0.5
In Number of Theoretical Plates (N) = 16 (tr/w)2. where tr: retention time, and W: peak width. My Question is here in formula what is 16.Can u explain briefly? ---Veerabhadrarao.M
3 Answers Micro Labs, Richter Themis, Sun Pharma,
what is a differance between assay by HPLC and assay by titration?
Many times I don't got a caffeine peak in calibration of hplc using guard column ❓