What is the difference between HPLC and GC?
Answers were Sorted based on User's Feedback
Answer / kanakadurga
In Hplc the principle is adsorption it is applicable for
separation of non volatile solutions.
where as in Gc the principle is partition it is applicable
for separation of volatile compounds.
Is This Answer Correct ? | 43 Yes | 4 No |
HPLC:mobile phase is liquid and stationary phase solid(silanes)
L=max 250 mm
Separarion:Based on adsorption
GC: mobile phase is gas and stationary phase liquid coated solid(silaxoneas)
L=30M
Separarion:Based on partition
Is This Answer Correct ? | 1 Yes | 0 No |
Answer / manisha
In hplc sample is partitioned between 2 phases and it is applicable for non volatile compounds whereas in cg adsorption phenomenon is taking place and mainly useful for volatile compounds.
Is This Answer Correct ? | 10 Yes | 11 No |
In dissolution test s1 stage we are getting one bowl 40 % out of 6 bowls what is the root cause you find and how proceed next step? ( Q is 80%)
why require to add enzymes in capsules dissolution only? is it require to tablet also?
which detector is used in IR
why hydrogen and zero air used for flame ignition in gc?
why use k2cr2o7 , kcl h2so4 in uv calibration
same sample on two preapration gives differnt result during hplc analysis
[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?
how to decide for one product require water content or LOD?
IN DISSOLUTION WE ARE USING A LETTER 'Q' WHAT IS MENT BY Q
5 Answers Reddy Labs, Torrent Pharma,
can we use the same detector in HPLC as well GC and what could be the differences we can find in the final chromato graph in any aspects?
what is stability study ? why it is needed and how itis done
What is the Baseline in HPLC.