how to hplc calibrated
- 4 Answers
- 50985 Views
- Dr Reddys, Glochem, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
HPLC is calibrated by the following parameters:
1) Pump calibration
2) Detector Linearity
3)Injector Precision and carryover test
4) Wavelength accuracy by using caffeine and pyrene solution
5) Determine the column oven temp. and sample compartment temp
6) Gradient test
7) Detector noise
| Is This Answer Correct ? | 128 Yes | 13 No |
Answer / ajinkya p.
In HPLC there must calibrations for Pump, Detector,injector
Pump :- Should calibrated by checking flow accuracy test, Pressure holding test.
Detector :- Should calibrated by checking Linearity test, Detector wavelength accuracy test
Auto sampler :- Should calibrated by Injector Accuracy test,
Carryover test, Injector precision test, carry over test
| Is This Answer Correct ? | 48 Yes | 2 No |
Answer / kanakadurga
calibration parameters are
1.Flow accuracy
2Temperature accuracy
3Wave length accuracy by using caffeine and erbium perchlorate
4Linearity of detector and injector
5.Carry over test
6.Drift and noice
| Is This Answer Correct ? | 37 Yes | 6 No |
Answer / b.ganesh
calibration parameters are
1.Flow accuracy
2Temperature accuracy
3Wave length accuracy by using caffeine and erbium perchlorate
4Linearity of detector and injector
5.Carry over test
6.Drift and noice
7.Gradient progromme accuracy
8.injector precision
| Is This Answer Correct ? | 3 Yes | 1 No |
what is the significant of pH in HPlc?
what is the meaning of PURITY AND POTENCY .what is the difference between PURITY and POTENCY
What is column dead volume?
Ph meter can show more than 14 ph reading? Why ph range in between 1 to 14 only?
What is Impuriteis?
why ph electrode is storing in KCL solution?Why the pH max is 14.0?
Home heating oil or furnace oil what is made from? Can (base oil plus diesel/kerosen) = home heating oil?
Retention time & Relative Retention time Definition & between difference? How to Calculate response factor(RF) & relative response factor(RRF)?
Can you please give a suitable definition and an example for understanding the words purity and potency.
6 Answers Cipla, Malladi Drugs and Pharmaceuticals, Reddy Labs,
why has inject 6 injection in Releted compounds
How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
How to know the estimated LOQ concentration in ppm
Organic Chemistry 
