Why we are performing wavelength accuracy at lower and
higher wavelengths in HPLC calibration
Answers were Sorted based on User's Feedback
Answer / tushar
this is because we can check absorbance in respective range
of minimum and maximum wavelength to get desire peak ,
retention time.
Is This Answer Correct ? | 14 Yes | 3 No |
Answer / bhalchandra farde anuh pharma
We are perform wavelength accuracy test in lower and higher
wavelengthe b'coz of responce of compounds depend on the
wavelength max therefore it is necessary to ensure the
accuracy of the wavelegth set during analysis to produce
consistent result in both range.
Is This Answer Correct ? | 4 Yes | 0 No |
Answer / d.thirupathi
The Uv detector range is 200nm to 400nm so that insted of
doing wave lenght accuracy at each level we are doing Lower
and higer wave lenghts so that it will cover all the uv
range.
Is This Answer Correct ? | 5 Yes | 1 No |
In HPLC what is Single point threshold, peak purity index and minimum peak purity index? what should be consider for pure peak.
a bottle of concerntrated HCL (36.5) was labelled as 37% (w/w) and density 1.19m/l.What is hcl molarity?
Calculate the water content in Di Sodium Tartrate (DST) based on molecular weight?
what is peak purity? how may types of methods to determine peak purity?
why the most API molecule are in the hydrochloride form?
What is the difference between qualitative and quantitative analysis (hplc)
For Dissolution test why we are Performing 6 Bowls why not more.any were mentioned or in what basis we are performing? --------Veerabhadrarao.M
2 Answers Lee Pharma, Micro Labs,
What is basic difference between method validation,process validation
Why we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?
In Analytical method development related substance method when did follow w/w method when did follow area normalisation method
what is difference in ods I ods II is this is realy from sorbent / packing characteristics or from other means
Why do we use KMnO4 in the test of control of obsorbance ? and why do we take specific quantity i.e 57-63mg?
0 Answers Reddy Labs, Vimta Labs,