In method developement or in any analysis if we use small length column for an

In method developement or in any analysis if we use small
length column for analysis what happen then ? why ?

Question Posted / tushar

3 Answers
7975 Views
I also Faced
E-Mail Answers

Answers were Sorted based on User's Feedback

In method developement or in any analysis if we use small length column for analysis what happen t..

Answer / kishore

retention times, analysis run times, back pressure will
reduced.

May mix closely eluting compounds

Is This Answer Correct ?

11 Yes

0 No

In method developement or in any analysis if we use small length column for analysis what happen t..

Answer / dharmesh bhatt

the question is not very specific, however, if one can use the shorter column for method development, they can optimize the method without sacrificing peak resolution. the theoretical plate and pressure will depend on the type of column & instrument one can select for analysis.
if the shorter column is used for analysis, it will shorten the analysis time and the resolution will sacrifice but again this will depend on the column and instrument.

Is This Answer Correct ?

0 Yes

0 No

In method developement or in any analysis if we use small length column for analysis what happen t..

Answer / qcprashant

Resolution and theoretical plate count will also decrease

Is This Answer Correct ?

0 Yes

1 No

Post New Answer

More Analytical Chemistry Interview Questions

analytical method validation require to with respect to release specification or shelf life specification?

0 Answers

What is difference between Chromatographic purity and Related substances analysis?

3 Answers

what is principle of XRD?

3 Answers Cipla,

Why chromic acid wash is not recommended by USP for glassware cleaning?

1 Answers Lupin,

What is the composition of K.F

6 Answers IPQA,

why KF factor is between 4.5 to 7.5 mg/ml ? why ? why sometime pyridine is used in analysis like in raw material solvent like acetone etc?

11 Answers Aurobindo, GVK, Sohan Healthcare,

what is dissolution What is unit of KF? what is the significant of pH in HPlc? WHY POTASSIUM HYDROGEN PTHALATE IS USED FOR PERCHLORIC ACID? What is difference btw UV detector & RID detector?

1 Answers

what is rt and rrt? how we find rrt? relation between rt and rrt/

1 Answers Cadila,

what is turbidimetric titration?Give 2 examples.

0 Answers Laurus,

Why we are having specification 100% above in case of assay as there is only 100% of compound?

2 Answers MSN Pharma, Nectar Lifesciences,

what is the film thickness in ftir

8 Answers

4. Describe the operation of the Craig apparatus. Chapter.3 Equilibrium Processes in Separations

0 Answers

For more Analytical Chemistry Interview Questions Click Here