Why we use Uracil for HPLC calibration in Detector wave
ength accuracy test?
Answers were Sorted based on User's Feedback
Answer / poojitha
uracil is a primary standard and shows maximum absorbance
at 260nm
Is This Answer Correct ? | 30 Yes | 9 No |
Answer / dhayasiva
Uracil is used as void volume indicator, Hence it is not
retained in HPLC column
Is This Answer Correct ? | 15 Yes | 7 No |
Answer / gangi reddy
Since it is a standard, Its a unretained substance in the
HPLC Column. No column is required for its elution.
Is This Answer Correct ? | 4 Yes | 4 No |
Answer / mr. bujji reddy kanchi
Since it is a standard, Its a unretained substance in the
HPLC Column. No column is required for its elution.
Is This Answer Correct ? | 5 Yes | 8 No |
Answer / flamingo rabale adl
1.What is stability indicating assay/ RS method ?
2. What is descriminatory disolution medium ?
Is This Answer Correct ? | 6 Yes | 23 No |
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hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
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