How much carbon loading in nomalphase columns?
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Answer / ajinkya
it contain only silica, and in reverse phase c8 and c18
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Answer / umesh chand
Normal phase column have no number of carbon ,its having silica gel particle coated with cellulose (3,5-di methylephenylcarbamate) and amylose (3,5-di methylephenylcarbamate)
like CHIRALPAK-OJ-H,CHIRALPAK -IA ,IB ,IC and etc.
'' polar stationary phase and nonpolar mobile phase'' use in normal phase chromatography
but carbon having hydrophobic nature, therefor we can't carbon loading in normalphase column.
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