how to correct peak when peak comes broader in shape?
How to do calibration of HPLC?
Answers were Sorted based on User's Feedback
Answer / satish
if peak is broad then use dilute solution of triethylamine to sharpen the peak
| Is This Answer Correct ? | 5 Yes | 1 No |
Answer / chanti1984
1. ENHANCE THE COLUMN OVEN TEMPARATURE, REDUCE THE SAMPLE
LOAD N COLUMN AND CHANGE THE DILUENT
2. URACIL/CAFFIENE SOLUTION ARE USING FOR CALIBRATION OF
HPLC WITH THEIR RESPECTIVE WAVE LENGTH
| Is This Answer Correct ? | 1 Yes | 2 No |
Tell me something about Vitamin A test method by HPLC
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