how to correct peak when peak comes broader in shape?
How to do calibration of HPLC?

Answers were Sorted based on User's Feedback



how to correct peak when peak comes broader in shape? How to do calibration of HPLC? ..

Answer / satish

if peak is broad then use dilute solution of triethylamine to sharpen the peak

Is This Answer Correct ?    5 Yes 1 No

how to correct peak when peak comes broader in shape? How to do calibration of HPLC? ..

Answer / chanti1984

1. ENHANCE THE COLUMN OVEN TEMPARATURE, REDUCE THE SAMPLE
LOAD N COLUMN AND CHANGE THE DILUENT
2. URACIL/CAFFIENE SOLUTION ARE USING FOR CALIBRATION OF
HPLC WITH THEIR RESPECTIVE WAVE LENGTH

Is This Answer Correct ?    1 Yes 2 No

Post New Answer

More Analytical Chemistry Interview Questions

In dissolution test s1 stage we are getting one bowl 40 % out of 6 bowls what is the root cause you find and how proceed next step? ( Q is 80%)

3 Answers   Reddy Labs,


why phenolpthalin gives pink colour when we titrate aganist sodium hydroxcide & phenolpthalin gives colourless titrate aganist hydrochlric acid which is un burate

1 Answers  


If vendor having more imp than monograph then how to proceed? and how to set spec?

0 Answers  


Plz explain the formula in uv calibration =Absorbance of the sample ( x ) x 10 x 1000/Weight of potassium dichromate taken in mg

1 Answers  


acceptance criteria for ph meter, hplc detector,pump,injector,oven when we calibrate.

3 Answers  






How to establish relative response factor for hplc. Why it is required?

0 Answers  


Describe 5 components of SFC ?

1 Answers   College School Exams Tests,


what are risk assement in the analytical qbd?

0 Answers  


injection volume accruacy test i calibration of hplc

3 Answers   HLL,


how to develop the icp ms method? Application of icp ms?

0 Answers  


how to calculate elemental impurities?

0 Answers  


The boiling point of 1,3 butadiene is -4.4 DC & the boiling point of Pentane is 36.1 DC. Then how come Pentane elute before 1,3 BD in GC analysis?

1 Answers  


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (342)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1387)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)