Interested to Buy Any Domain ? << Click Here >> for more details...
Why triethylamine and o-phosphoric acid used while setting
pH of mobile phase during HPLC Analysis?
- 6 Answers
- 33893 Views
- Hapten Healthcare, Pharmaceutical, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / g.g. krishna
Triethylamine: To reduce tailing of basic analytes
Phosphoric acid: To suppress the ionization of acidic analytes
| Is This Answer Correct ? | 23 Yes | 2 No |
TRI ETHYLAMIINE (AND) ORTHO-PHOSPARIC ACID ARE
USED pH ADJUSTMENT PERPASE
BECOSE
1)GOOD RESOLUTION
2)PEEK SHAPE
| Is This Answer Correct ? | 12 Yes | 3 No |
Answer / venugopal
These are weak acid and weak base compared to
HCl,sulphuric acid, NaOH.Generally the salts used in
preparing the buffers are sodium phosphate/potassium
phosphate.Hence these reagents are added.
| Is This Answer Correct ? | 9 Yes | 1 No |
Answer / valla priyanka reddy
triethylamine is added for good shape of peak
| Is This Answer Correct ? | 14 Yes | 7 No |
Answer / murali krishna
Mainly Triethyl amine is used becauseof the peak Tailing and shape of the peak
| Is This Answer Correct ? | 4 Yes | 1 No |
What if impurity area in control sample coming more as compared to LOQ level of impurity ?
what is stability study ? why it is needed and how itis done
what is difference between drug products and drug substance?
How to calculate lamp energy in hplc?
Many times I don't got a caffeine peak in calibration of hplc using guard column ❓
What is the difference between chromatographic purity and related substances?
difference between record & document ?
What is control room temperature and which guide line says?
what is lod and loq ?,why use k2cr2o7 , kcl h2so4 in uv calibration ?,why use benzophenone & caffene acetone in hplc calibration ?,what is leading peak in hplc ?why we do the calibration of limit of stry light in hplc & uv ?
What is similarity factor and its use in api strength?
What is the difference between Optical rotation and Spcefic optical rotation
what is the difference between isocratic & elution technique?
Organic Chemistry 
