What is the b/W dead volume and dwell volume ?
Answers were Sorted based on User's Feedback
Answer / falguni patel
Dead volume: It comprises the volume of HPLC system between
the point of injection to the point of detection, excluding
the part of column that contains packing.
Dwell volume: It includes the volume between pump and
column. in low pressure gradient it may be 1 mL & in high
pressure gradient it is to less.
Is This Answer Correct ? | 14 Yes | 2 No |
Answer / aks
Dead Volume:Volume between injector to detector excluding
column volume (and also called as extra column volume)
Dwell Volume:Volume form the point of mixture to the column
entry (Also called as system delay volume)
Is This Answer Correct ? | 10 Yes | 0 No |
What is Spliit and Splitless in Gas Chromatography nad How does it work?
How to prepare 50 ppm ammonia solution form 25% ammonia solution?
What is the difference between uplc and hplc
4 Answers AAU Assam Agricultural University, Hetero,
Can we interpret accuracy from linearity in method validation?
what is the purge flow & how to calculate
if tech transfer fails whay require to do?
what is lod and loq
how can I make the copper get white bye any salt or acit?
what is the difference between mcg and ppm?
what r the parts calibrated in gc
What is the principle of GC/HPLC ?
4 Answers BioCan, Biocon, Megafine Pharma,
In chromatograpbic purity 90% is there, in assay 70% is there. How will you prove remaining 30%.