In HPLC Chromatographic separation Resolution equation is
Rs = 1.18 (tR2 –tR1)/wh1+Wh2,
Where, tR2 and tR1 is run time of eluent ratios of the
distances from the point of application to the centers of
the spots and the distance travelled by the solvent front
from the point of application (retardation factor), and
Wh1and Wh2 , is peak width of at half height.
What is 1.18 ?
Answer / bhavin
In a peak with Gaussian distribution, the peak width is W = 4 σ (where σ is the standard deviation) and the peak FWHM is W0.5h = 2.354σ. hence 1/2*2.354=1.177=1.18
Is This Answer Correct ? | 0 Yes | 0 No |
how you establish the LOQ?
Describe your experience with quality assurance and quality control analyses?
11 Answers Aurobindo, Dr Reddys, MSC, Reddy Labs,
iam using ph instrument lab india make.3ponit and 5point.in calibration time 3point slope will come 98.89and 96.87.that limit is 85 to 105.what basis slope will come.any calucalation between them.5point wiicome slope 99.89?why 3p0int came 2 values
mode of absorption in alimentary canal?
How to calculate the maximum loaded sample concentration in HPLC column?
What is similarity factor and its use in api strength?
only one end point is possible for citric acid and we get 2 end points for di acid why?
What are the factors effect peak shape in HPLC ?
2 Answers College School Exams Tests,
Data was integrated with RRT CONCEPT OR Overlay with SSTchrmatogram concept which is better?
what are risk assement in the analytical qbd?
Why irmoisture balance is used for corrugated box moisture testing
what is difference between zero air gas and o2 gas
1 Answers IPCA, Vasudha Pharma,