What is use of LOD and LOQ in validtion and give range and in calculation why we use 3.3* LOD here why we use 3.3
- 2 Answers
- 5546 Views
- Dr Reddys, IPCA, Reddy Labs, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / nikkosthiru@gmail.corai12
LOD is Limit of detection. We can dedect the peak but cant quantify
LOQ Limit od quaantification. We quantify also this is known as reporting thereshold.
During the RS analysis Below LOQ level we cant included in the total impurities calculation.
LOD and LOQ Determination three methods
1.S/N Ratio method
3 or 2 ratio for LOD
NLT 10 ratio for LOQ
2. Slope Method
Plot the linearity for active and impurities through serial dilution at lower level
Formula for LOD
3.3*Slope/Std.dev
Formula for LOQ
10*Slope/Std.Dev
3. Visualization method
It will be using for only identification and TLC Method
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / kovida
LOD and LOQ are the smallest amount of the particular compound that can by detected and quantiified by using the developed HPLC method.
The signal to noise ratio is that minimum amount which when injected in HPLC it gives minimum detectable peak area.
The value of amount at this point is multiplied by 3 to get LOD
and by 10 to get LOQ value. In short LOD is the thrice value of minimum value and LOQ is ten times of minimum value that can be injected in HPL
| Is This Answer Correct ? | 1 Yes | 2 No |
In the test for control of absorbance in UV calibration why do we use only potassium dichromate and what is purpose of taking a specified amount of 57.0-63.0mg?
in dissolution in one tablets two molecule one is losartan & second is HCTZ losartam is complies in s1 stage & hctz is complies in s2 stage then how to report result losartan s1 stage result or losartan s2 stage result
IN DISSOLUTION S1 STAGE 1 BOWL GOT 52%(Q POINT IS 80%)WHAT WE HAVE DO
What is the difference between specified impurity and Identified impurity
Why using Holmium oxide in UV calibration
8 Answers Dr Reddys, Matrix, Nakoda Chemicals, Orchid,
HOW TO CALCULATE THE MASS OF UNKNOWN IMPURITY IN LC-MS? AND WHAT IS THE ROLE OF FRAGMENTATION IN LC-MS ?
What is the Calcultion of S/N Ratio.
How we fix the validity period of a Normal solution and re-standardization due date
how to develop the icp ms method? Application of icp ms?
what is role of pH in mobile phase
3 Answers Cipla, Dhanuka Laboratories,
what is difference between GC & GC-HS
In HPLC when we use Isocratic method, Gradient method in hplc?
Organic Chemistry 
