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Why Urasil and Caffeine use for HPLC wavelength accuracy
calibration. which is most suitable, Why? other than these
which can be used?
- 7 Answers
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Answer / hemadri
Because it is stable in any condition . Easily availability in market. It will give sharp peak and run time also less.
| Is This Answer Correct ? | 13 Yes | 3 No |
Answer / suresh polisetti
bcoz these r gives the both abs max & min in the UV Region which means max= 205,273,min=245,
| Is This Answer Correct ? | 10 Yes | 0 No |
Answer / manish
caffeine and urasil solution is more stable at rt and show
multiweve length .
| Is This Answer Correct ? | 4 Yes | 1 No |
Answer / rishabh
anthracene and holomium oxide can also used for wavelength accuracy detector
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / mahendra
Because it give dual wavelength absorbance like 245nm 350 nm
| Is This Answer Correct ? | 5 Yes | 12 No |
Answer / chemistry
Because uracil And Caffierne Shows The absorbance Range in
245nm And 340nm.
| Is This Answer Correct ? | 0 Yes | 7 No |
Answer / gaurav oza
caffeine and urasil does not retain in HPLC. They are
generally used for measuring the dead volume or void volume
, so both are used for calibration of HPLC column
| Is This Answer Correct ? | 2 Yes | 10 No |
Dear sir, This is R.Kadambavadani working in a pharma industry. while doing RS analysis of ibuprofen API, unknown peak eluting some times in the 0.6 Min RT. Kindly send me what is the exact reason behind that. also am using ACN the mobile phase.
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