Answer / sridhara h s

1) Check the for loose fittings, check pump for leak, salt
build up in pumping area,
2) If mobile phase contamination or detoriation,
3) If detector is not working properly - weak lamp detector,
4) Any air trapment in the system and as well as in mobile
phase,
5) Air bubles in detector - make purging proprly,
6) If cloumn is leaking silica or packing material passing
through detector.

Answer / rajib hazarika

properly washing or cleaning of the system with hot water followed by 70% IPA is sufficient to overcome the problem of Noise and Drift. (provided no leakage in system and use of freshly prepared mobile phase with proper sonication.)

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