what is the significant of pH in HPlc?
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selecting the pH of M.P. is important to get credible
retention time and is based on the pKa value, polar or
non-polar nature of the analyte.
| Is This Answer Correct ? | 33 Yes | 1 No |
Answer / roshan bhaskar
pH can change the hydrophobic character of the analyte. For this reason most methods use a buffering agent, such as sodium phosphate, to control the pH. Buffers serve multiple purposes: control of pH, neutralize the charge on the silica surface of the stationary phase and act as ion pairing agents to neutralize analyte charge.
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Answer / pawan gupta
pH is the main role in M.P. pH depend retention time &peaks Sharp
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