Answer Posted / balaram naidu.m

HPLC in mainly two types of columns is used
These are (1)Normal phase columns
(2)Reverse phase columns

C18 And C8 COLUMNS both are reverse phase columns.
C18 columns is highly polar columns compare with C8 columns

C18 Column Structure is OH-Si-C18 linkage

C8 Column Structure is OH-Si-C8 linkage

These columns is depend up on the compound natchere

ODS means OCTA DECYLE SILANE. This types of columns
is used mainly acidic nacture compounds.

BDS means BASE DEACTIVATED SILANE. This types of columns
is used mainly basic nacture compounds.

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