which is called as mouse antilopecia factor?1 2379
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
Which organ and subcellular site are most important for fatty acid biosynthesis?
Why are right-handed helices more stable than left-handed helices?
mutarotation is observed when phenol and pyridine both are present but phenol or pyridine alone cannot show mutarotation-explain why?
What is zone electrophoresis?
which fat soluble vitamin has co-enzymic function?
ano po ba ang bermuda triangle? ano po ang nandito? meron po bang mga naitala na aksidente sa gawing ito? ano po ang scientific question nito?
During a heart attack, blood flowing to the heart muscle is interrupted by blockage of a coronary artery. How would you expect the metabolism in the heart to change?
How are free energy, equilibrium and spontaneity related to each other?
Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.
How are reaction rates dependent upon free energy changes?
How do hydrophobic and hydrophilic residues arrange themselves in globular proteins?
How does hplc differ from normal column chromatography, and what are its advantages?
Hi, I am venkat. I am done with M.Sc Medical Biochemistry in the year 2007. I got rejected 3 times at US consulate, and the reason they say is why are you going agian for PG when you have already done PG, and they are not ready to get convinced only on this issue.
How are free energy changes related to LeChatelier's Principle?