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If you have given an unknown compound how you develope a
hplc analysis method for anlysis?
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Answer Posted / ashutosh bhatt
various steps should be perform
1. solubility
2.pka value determination
3.uv lemda max determination for detection
4.selection of organic solvents for mobile phase
5.selection of analytical column
6.temperature effects
7.organic solvents ratio selection.
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