If you have given an unknown compound how you develope a hplc analysis method f

If you have given an unknown compound how you develope a
hplc analysis method for anlysis?

Question Posted / atul

4 Answers
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If you have given an unknown compound how you develope a hplc analysis method for anlysis?..

Answer / atul

First check the solubility and uv of that compound and then
we should decide the which method is suitable for tha analysis.

Is This Answer Correct ?

38 Yes

5 No

If you have given an unknown compound how you develope a hplc analysis method for anlysis?..

Answer / ashutosh bhatt

various steps should be perform
1. solubility
2.pka value determination
3.uv lemda max determination for detection
4.selection of organic solvents for mobile phase
5.selection of analytical column
6.temperature effects
7.organic solvents ratio selection.

Is This Answer Correct ?

15 Yes

2 No

If you have given an unknown compound how you develope a hplc analysis method for anlysis?..

Answer / dheeraj pandey

FIRST WE SELECT THE SOLUBILITY OF THE UNKNOWN SAMPLE AND
MAKE THE DILUTION AND OBSERVE THE ABSORBANCE IN UV AT
SPECTRUM MODE AND SELECT THE LAMDA MAX AND ON PHOTOMETRIC
MODE OBSERVE THE CHRMATOGRAM AFTER THAT WE SELECT A MOBILE
PHASE ON THE SOLUBILITY OF SAMPLE AND DEVELOPED THE METHOD

Is This Answer Correct ?

4 Yes

1 No

If you have given an unknown compound how you develope a hplc analysis method for anlysis?..

Answer / prathyu.chunduru

first find out the solubility of the drug and then pka of the drug and absorbtion maxima of the drug on uv.And then start the trails by using different ratio of aq and organic phase.If we dont get sufficient parameters then go foe the buffer and it should have a ph of +/- 1 of pka of the drug.Then go for the trails.

Is This Answer Correct ?

4 Yes

1 No

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