If we are getting split peak in Chromatogram,what we can do?
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Answer Posted / satyasri
if u observe peak splitting,first u go to change(reduce) injection volume(means volume over load)...dn u go for buffer srength(means conc)...dn u go for pH of the buffer...dn only u go for checking of column performance(means whether u hv to check column was washed properly from previos one)........k
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