How will you select mobile phase in HPLC Method Development
Answer Posted / maulik
based on the pka value of compound. if ur compound have pka
is about 3. u can select ur mobile phase about ph 5-14.but
due to the less column selectivity in basic media u can
select 5-8 pH. preferred by the different salt buffering
capacity.
Is This Answer Correct ? | 19 Yes | 5 No |
Post New Answer View All Answers
What is the formula for relative diffrence for standard solution in solution stability in validation?
In GC calibration,what is the procedure to calibrate the TCD (thermal counductivity detector)suggest ?
what is definition of validation? which components are followed give detail?
What is the use of tlc and hplc? And when and where use?
can we use the same detector in HPLC as well GC and what could be the differences we can find in the final chromato graph in any aspects?
how will you do the prep for unstable componds?
what is the purge flow & how to calculate
Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?
What is the acceptance criteria for moisture balance when calibrated with sodium sulfate
How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.
what is the difference between potentiometric titration and karl fischer titration?
What is viod volume and peak purity in HPLC?
In HPLC Calibration, On which basis RSD Limit of noise test is fixed (NMT 33.0 % )
How do we fix the sample concentaryion in hplc method development?
what type of question will ask in the interview of lab chemist.