How you will select the runtime in HPLC for a drug in
method development.
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Answer Posted / sarma dvv
In the method development, we have to extend the run time 7-
8 times more with analyte retion time. we have to check any
other peaks are eluting. In that run nopeaks are eluted
means accordingly we can fix the run time.
Generally for isocretic 3times more with resect to analyte
peak.
For Gradient we have to extend the organic concentration
maximum possible.
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