What is calibration procedure of gredient HPLC pump
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Answer Posted / prerna
Gradient Programme for port A and C :
00 : 100
00 : 100
25 : 75
25 : 75
50 : 50
50 : 50
75 : 25
75 : 25
100 : 00
100 : 00
Above programme also run for port B and D
An increasing stepwise piramid will appear. Integrate that
peak and also split each step of the peak.
Find out its height and area of height
By using the formula ( which I don't remeber ), mean flow
of solvent at each step.
Flow : Limit
25 (24-26)
50 (49-51)
75 (74-76)
100 (100)
If the observed value is within the acceptance limit then
that pump passes the test.
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