In case of HPLC coloums what is the difference Between C8 &
C18 coloums & how can we select difference type of coloums
like Inertial ODS , Hypersil BDS , ODS etc.?
Answer Posted / pharmainterview
C8- C8 Carbon chain is attached to silica
c18- C18 Carbon chain is attached to silica.
There are many manufacturer who will prepare c8 or c18
column. Even though the basic concept is same for all,
there are some differences like
1.pore size
2.particle size
3.free silanol
4.end capping
5.silica purity etc
Based on our sample properties and suitability we can selct
a column after different trials.
Regards
pharmainterviewquestions.blogspot.in
Is This Answer Correct ? | 9 Yes | 1 No |
Post New Answer View All Answers
calibrtion procedue of LC-MS
please explain about aluminium hydroxide assay
How will u perform degradation study in detectors other thann PDA in HPLC Methodology
CAN WE DETERMINE THE PEAK PURITY IN GAS CHROMATOGRAPHY ANALYSIS?
how you start RS method development when for new product?
What is the difference between Discriminating media and DPDM(Dissolution Profile with Different Media)
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18
on saturation solubility study data how we can find out the bcs class of drug?
In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.
What is the main difference between ODS1 and ODS2 Hplc column.
why octanol used to determine the partition coefficient ?
Why do we use KMnO4 in the test of control of obsorbance ? and why do we take specific quantity i.e 57-63mg?
what is mean by extactable and leachable study?
Principle of single pan analytical balance
Why only 1.2 million lux hours require in photostability study?