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In case of HPLC coloums what is the difference Between C8 &
C18 coloums & how can we select difference type of coloums
like Inertial ODS , Hypersil BDS , ODS etc.?
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Answer Posted / pharmainterview
C8- C8 Carbon chain is attached to silica
c18- C18 Carbon chain is attached to silica.
There are many manufacturer who will prepare c8 or c18
column. Even though the basic concept is same for all,
there are some differences like
1.pore size
2.particle size
3.free silanol
4.end capping
5.silica purity etc
Based on our sample properties and suitability we can selct
a column after different trials.
Regards
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