Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...

how peak can be sharp in hplc when it is come flat

Answer Posted / sasikumar.r

1. increase the concentration of organic phase. i.e.,
increase the ratio of organic phase in mobile phase

2. Add little amount of peak modifiying agent TRIETHYLAMINE

3. just REVERSE the column

Is This Answer Correct ?    15 Yes 5 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

Why Ethanol is Used for Standardization of GC Head space?

4156


How to establish relative response factor for hplc. Why it is required?

1151


Tell me about analytical method validation in QC

6126


CAN WE DETERMINE THE PEAK PURITY IN GAS CHROMATOGRAPHY ANALYSIS?

1149


If we do accuracy at same concentration at which linearity planned,what is the need to do linearity separately?

2240


In GC calibration,what is the procedure to calibrate the TCD (thermal counductivity detector)suggest ?

3119


how many types of balance calibration test?

2249


how will you do the prep for unstable componds?

1973


How to calculate coreletion coefficient

1635


What is intact assay method development.....?

2557


how require to select dissolution media? what is discrimination?

744


For titration in anhydrous media with perchloric acide, if lack of titrator, Which indicator is been used for replacement. How calculate pH of test solution to choose suitable indicator?

2263


WHAT IS THE PURPOSE WE USE HCL, CHOPPER, BURNER, MONOCHROMATOR in AAS? PLEASE EXPLAIN BRIEFLY

2565


which are the diffrent batches in the pharmaceuticals?

768


give clarity of linearity and range in method validation

2379