how peak can be sharp in hplc when it is come flat
Answer Posted / sasikumar.r
1. increase the concentration of organic phase. i.e.,
increase the ratio of organic phase in mobile phase
2. Add little amount of peak modifiying agent TRIETHYLAMINE
3. just REVERSE the column
| Is This Answer Correct ? | 15 Yes | 5 No |
Post New Answer View All Answers
how to set sample and standard concentration in RS method?
if you given one product then which tests you will perform?
effect of pore size, pore volume, partical size, column length, carbon load on retention time? what is carbon load? what is the use?
What is delay volume?
what is mean by peak intigrity?
why are measure gas flow " ml " in Gas chromatography
in which situation ion pair agent require to use?
What is the acceptance criteria for moisture balance when calibrated with sodium sulfate
How to fix the sample conc. in Related substances test by HPLC?
mode of absorption in alimentary canal?
Please tell me about the pH of Polycaboxylic ether is it in the 5-6 range ever or more than 6
why lactose has less charges when packed in steel containers when compared with packing of lactose in polyamide material?
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18
For standardization of volumetric solutions, acceptance limit for concentration is considered as 10% of nominal concentration and %RSD as NMT 0.2% (for appropriate no. of replicates). But , if %RSD has not met, what should be further action?
why digestion require in icpms?