Answer Posted / praveen kumar (ipca ranu)
Peak purity refers to the degree of separation between two peaks in a chromatogram, which is a graphical representation of the components in a sample as they elute from a column in HPLC.
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2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
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