Answer Posted / r.kadambavadani
The column stationary phase is also affected by pH. At very low pH (<2) the bonded stationary phase will be stripped from the silica support. At high pH (>8) the silica itself will be damaged by dissolution. At mid pH ranges (>5), any lone silanol groups (Si-O-H) present will act like an acidic moiety and will become ionized - increasing the tailing observed with analytes containing basic functional groups.
Is This Answer Correct ? | 1 Yes | 0 No |
Post New Answer View All Answers
why we use Dichlorobenzene.nitrobezine.t-butyldi sulhate.for calibration of gc Head space
How can I develope method of dissolution by HPLC OR UV
What is importance of pH in the Heavy metal test and How it effect on the test results?
using gradient pressure in gas chromatography are not ?using gradient pressure why
if rsd failed then what require to do?
how to calculate elemental impurities?
In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.
[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?
In the HPLC Calibration done wavelength accuracy done between 200nm-280nm .but not done remaining 300-400nm not done ?
which are the diffrent batches in the pharmaceuticals?
How we performed the force degradation for drug substance, is any specific guideline is available for each parameter(Acidic, basic, oxidation,heat)? what conditions you mentained for above parameters.
What is third generation HPLC Columns?
What is intact assay method development.....?
calibrtion procedue of LC-MS
we are performed SOR for a particular product the limit is -6 to -10.We face particular bathes it is not meeting the specification.we performed diffrent instrument diffrent analyst we are getting diffrent diffrent results.solvent is dimethyl sulfoxide what could be the reasion