What is the reason of RT change in new column v/s old column in HPLC
- 3 Answers
- 16688 Views
- Apex, I also Faced
- E-Mail Answers
Answer Posted / naresh
Regular usage of old column some stationary phase is loss inside the column compared to new column so compound retention inside the column varies between these two columns
| Is This Answer Correct ? | 7 Yes | 0 No |
Post New Answer View All Answers
why we are using hexane in calibration of number of drop per mL
Why to use propyl paraben in HPLC gradient calibration
What are the primary considerations in extractables and Leachables ?
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18
how a particular wavelength can be different for a particular compund while analysing by uv and by HPLC.
How to set analytical specification for combination products?
Which products will produce when acetic anhydride reacts with Ter-butanol, Sec-butanol, Iso butanol, Dimethylamine etc.
in dissolution if one bowl got 70 percent 2nd bowl got 80 percent and 3rd bowl got 90 percent then how proceed?
process of Diclofenac sodium,IP.
In which situation we require to analytical method validation of excipient?
How do we fix the sample concentaryion in hplc method development. What is the basis?
function of detecter in hplc ,gc and spectroscopy? function of carrier gas in gc?
if you given one product then which tests you will perform?
WHAT IS THE CRITERIA FOR SELECTION OF TIME OF DISSOLUTION AND THE MEDIUM OF DISSOLUTION?
CAN WE DETERMINE THE PEAK PURITY IN GAS CHROMATOGRAPHY ANALYSIS?
