How we can analysed unknown sample by hplc....
Answer Posted / ravindra
First take ir of that sample and judge the functional
goroups attached to that compound and also structure of
that compound.
then check solubility.
then UV scan with known concentration and find lymda max.
then selection of column.
first try with regular solvent like water, acn,
methanol.and then go to the buffers of different pH.near to
its pka value.
also different coloumn.
Is This Answer Correct ? | 2 Yes | 0 No |
Post New Answer View All Answers
in sampling of raw material which side is pasted sampled labels
is it nessesary to do solution stability for 7 days?
Why a1% value is used for some product ? What is the criteria for selection a1% value ?
in api coa contains only process impurities and in product coa contains degradation impurities?
WHAT IS THE PURPOSE WE USE HCL, CHOPPER, BURNER, MONOCHROMATOR in AAS? PLEASE EXPLAIN BRIEFLY
what is impurity profile. how to interpret this impurity profile to a drug product or drug substance.
Which parameters require to do in tech transfer?
How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.
Why use only methanol,used determinationof moisture content not use other solvents
What is viod volume and peak purity in HPLC?
Why irmoisture balance is used for corrugated box moisture testing
if you have given one product then how you determine the impurity in that?
What is shaking level in GC?
what is different when impergnated silica plates are used in separation of azo dyes using column chromatography?
give clarity of linearity and range in method validation