today i am doing calibration of HPLC. when i inject the
blank it was going on -ve.(bleow then zero) why?

Answer Posted / sudheer kumar m

may be mobile phase was not stable in that particular
method. so condition the column with mobile phase and re inject.

Is This Answer Correct ?    2 Yes 1 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

why does used dry caffien in HPLC calibration?

1570


why require to do water content for drug product?

319


How doing qbd practically?

434


how require to set assay concentration for standard and sample?

371


Why we select scan range from higer wavelength to lower wavelength in uv visible spectroscopy and ftir spectroscopy ? .

462






in each media we require to use SLS? how to proceed?

301


Why sometime potassium ortho phosphate mix with acetone use for sample preparation for hplc

984


if you have given a blank water how you analyse by general analytical techniques?

1510


If we have 5 strength which is not dose proportinate and excipients also diffrent in each strength then how we can proceed for Force degradation? and excipient are same but not dose propotinate the how FD?

334


What is the use of tlc and hplc? And when and where use?

664


when we talk about change in entropy we consider total entropy means of surround +entropy of system but when we talk about change in Gibss free energy we consider entropy of system.explain with reason.

1524


Tell me something about Vitamin A test method by HPLC

2209


why we use Dichlorobenzene.nitrobezine.t-butyldi sulhate.for calibration of gc Head space

1783


What is stray light in uv what are units for stray light

1026


AT WHAT CONDITIONS WILL YOU ANALYSE A SAMPLE,WHICH ARE PRESERVED AT OTHER THAN THE AMBIENT CONDITIONS?(ie COLD STORAGE SAMPLES BELOW 20* CENTIGRADE?

1606