Answer Posted / satheesh kumar
Post run in hplc comes under the gradient programme
(Gradient elution).
Gradient Programme-:
Time(min) A% B%(Where-A% Buffer,B%-ACN)
0.01 80 20
10.00 50 50
25.00 20 80
30.00 20 80
35.00 80 20
45.00 80 20
In the above gradient Intially starts with Buffer,ACN in
one proportion,when the time incerases proportion changed
and finallty returns to original proportion is called Post
run or equilibrqtion time
Is This Answer Correct ? | 13 Yes | 2 No |
Post New Answer View All Answers
What is the acceptance criteria for moisture balance when calibrated with sodium sulfate
How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
about method developement in hplc
In IR spectra, generally a molecule vibrates without apply emr or ir light?
all types of questions asked at the cipla interview
what is mean by covalidation
How do we fix the sample concentaryion in hplc method development. What is the basis?
how to calculate elemental impurities?
What is dose dumping? why require to do?
in which situation ion pair require to use?
What is mean by PDR?
why are measure gas flow " ml " in Gas chromatography
on the basis of bcs class how to proceed the solubility?
In Dissolution Test why limit is define Q+5% what is the role of +5%.
why we use glass fiber filters use in some situation?