what is use of acetonitrile compare to methanol in RP-HPLC
Method devlopment
- 7 Answers
- 22021 Views
- Neuland, Orchid, I also Faced
- E-Mail Answers
Answer Posted / balaji
the uv cut oof value of methanol is 210 nm so we cant use
methanol near about lambda max of 200 nm. so prefer to ACN
& ACN is highly polar as compare to Methanol So provide
Better resolution for many compound and it has property to
form hydrogen bond so provide better selectivity.
Methanol viscosity more than the ACN. so ACN easly flow
through column
| Is This Answer Correct ? | 15 Yes | 1 No |
Post New Answer View All Answers
some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?
what is the different in Total ash, sulphated ash, acid insoluble ash, alcoholic or non alcoholic ash?
What is the difference between Discriminating media and DPDM(Dissolution Profile with Different Media)
What is rs test why we are performing rs test?
is it nessesary to do solution stability for 7 days?
for which product require to do content uniformity? what is limit of cu?
how much mass should be there in volumetric flask while in preparation of sample for assay?
give clarity of linearity and range in method validation
What is the difference between chromatographic purity and related substances?
Principle of single pan analytical balance
Why only 1.2 million lux hours require in photostability study?
what is mean by 40 in the dissolution basket mesh size?
in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?
Which are the diffrent grades of api in pharma?
could negative ions be produced by bombardment process in mass spectrometry?
