why using buffers in hplc inteed of water

Answer Posted / srinivas torati

in water some compounds didn't ionization thats why we
select buffers certain ph range in acid ph some compounds
ionization is good basic medium ionization less thats why
uv different in different ph's

Is This Answer Correct ?    3 Yes 2 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

IS THERE ANY EQUIPMENT TO CHECK AND CALCULATE THE POLARITY OF A LIQUID?

1989




1822


Please tell me about the pH of Polycaboxylic ether is it in the 5-6 range ever or more than 6

2113


Tell me about analytical method validation in QC

5562


what is mean by dissolution biowaiver study?

330






How can I develope method of dissolution by HPLC OR UV

1893


How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.

2050


In which situation we require to analytical method validation of excipient?

300


What is similarity factor?

731


How require to perform linearity as per ANVISA? What are the acceptance criteria?

726


what is the extinction coefficient for Indomethacin or at 319nm wave length.

3523


what is the origin to prepare standard operating procedure

1793


how to decide for one product require water content or LOD?

330


please explain about aluminium hydroxide assay

713


how pda detector works over uv?

297