How to know HPLC column performance?

Answer Posted / vijay muley

1) check the back pressure,
2) check Theoretical plates,
3) system suitability as per test requirement e.g. tailing
factor, symmetry, RRT, resolution etc.
all three parameters shows columns performance.

Is This Answer Correct ?    20 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

What is control room temperature and which guide line says?

1160


can i use hplc detector to uplc and why?

1815


mode of absorption in alimentary canal?

1922


in hplc chromatogram started from left to right and in uv spectrum started from left to right

471


2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.

1768






some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?

2101


How to set specification of assay, fisdolution and related substances?

368


in api coa contains only process impurities and in product coa contains degradation impurities?

324


What is the difference between purge septum flow and column flow in gas chromatography?

1840


what is diffrence between specificity and selecivity?

318


if you have given a blank water how you analyse by general analytical techniques?

1510


what is lod and loq ?,why use k2cr2o7 , kcl h2so4 in uv calibration ?,why use benzophenone & caffene acetone in hplc calibration ?,what is leading peak in hplc ?why we do the calibration of limit of stry light in hplc & uv ?

3189


iam usig ph buffers merk. manually how to prepare ?

1781


what is the difference between potentiometric titration and karl fischer titration?

2426


how to get accurate result for Residue on evaporation in purified water

1124