In the method validation proceedure,lod & loq calculation we use the formulae for LOD=3.3*SD/slope and for LOQ=10*SD/slope,why we use 3.3 & 10 in the formulae?
- 1 Answers
- 10096 Views
- I also Faced
- E-Mail Answers
Answer Posted / veeru01
LOD :- LOD is measured 10 times independent sample of Blank
and Student-t test of 10 times, Degree of freedom 9 (n-1)at
99% confidence label is 3.25 as per Student chart. Also
Ratio of S/N =3:1
LOQ:- LOD is regarded as the lower limit for precise
quantitative Measurement . LOQ defined as concentration
which would a produce a signal 10-12 time. At this point
standard deviation is small enough (Approximately 10-15%) so
that quantified value can be deemed reliable. Also Ratio
S/N= 10:1
| Is This Answer Correct ? | 7 Yes | 3 No |
Post New Answer View All Answers
What is the purpose of octyl silane columns?
how we can identify the impurity is coming below loq at transfering site?
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
is it nessesary to do solution stability for 7 days?
why octanol used to determine the partition coefficient ?
how to calculate elemental impurities?
HI,I CLEARED BOB CLERK EXAM. MY INTERVIEW WILL BE ON 9TH OCTOBER,2010.PLEASE SEND ME INTERVIEW QUESTIONS AND ANSWERS. THANK YOU.
in dissolution why pool sample needed? in which type of drug pool sample need?
what is the principle of UV Vis spectroscopy, AAS, ICP OES,ICPAES, ICP-MS and FTIR
Why only 1.2 million lux hours require in photostability study?
In performance Check of GC Why Hexadecane Peak is ConsideredÂ
How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?
please explian me about area normalisation method,diluted standard method,impurity vs impurity and which one has to be selected in method development
we are performed SOR for a particular product the limit is -6 to -10.We face particular bathes it is not meeting the specification.we performed diffrent instrument diffrent analyst we are getting diffrent diffrent results.solvent is dimethyl sulfoxide what could be the reasion
How require to develop GC method? how to select diluent, GAS, column selection and other chromatographic conditions?
