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why we use caffeine for hplc calibration?
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Answer Posted / bhagavanji nelakanti
Caffeine is a primary standard which is having good
qualities like very soluble in methanol, highly stable at
room temperature and given set of conditions also. It is
having high boiling point, cheap in cost, easily available.
It is easy to prepare and handle and give good responses
(lambda max) at 205nm and 272 nm, (lambda Min) at 244 nm. We
can identify the difference in response with changing of
single nm also(271-273nm).
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