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WHAT IS DIFFERENCE BETWEEN RRT AND RRF IN HPLC
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Answer Posted / naresh t
Relative retention time (RRT) is used to know where peaks apart from main compound elutes in HPLC analysis.
Where as Relative Response factor (RRF) comes into picture when our compound and impurities have different wavelength maxima. In this case impurities show either higher or lower response when the analysis is performed at wavelength maxima of main peak. So in quantification analysis the values obtained for impurities are not the actual values. In order to calculate the actual value of impurities' a correction factor is applies. this factor is called RRF
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