Why caffeine as a reference standard used for calibration
of HPLC?
Answer Posted / rohidas baban shinde
Because it shows maxima at the wavelength of 205 nm and 273
nm and shows and also shows minima at the wavelength of 245
nm. It is a Primary reference standard and it is avaliable
in the market very easily.It is cheap.It is non-degradable
and we can used it for longer period of analysis.
Is This Answer Correct ? | 11 Yes | 0 No |
Post New Answer View All Answers
If we have 5 strength which is not dose proportinate and excipients also diffrent in each strength then how we can proceed for Force degradation? and excipient are same but not dose propotinate the how FD?
give clarity of linearity and range in method validation
why sre you used Potassium hydrogen phthalate in standarisation of 1N NaOH and 0.1 N Perchloric Acid?
AT WHAT CONDITIONS WILL YOU ANALYSE A SAMPLE,WHICH ARE PRESERVED AT OTHER THAN THE AMBIENT CONDITIONS?(ie COLD STORAGE SAMPLES BELOW 20* CENTIGRADE?
In Dissolution Test why limit is define Q+5% what is the role of +5%.
effect of pore size, pore volume, partical size, column length, carbon load on retention time? what is carbon load? what is the use?
Did anybody have method for acetyl cysteine effervescnce tablet
What is a difference between potency and purity?
How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?
what is the difference between potentiometric titration and karl fischer titration?
How will u perform degradation study in detectors other thann PDA in HPLC Methodology
if instrument calibration fails then what require to do?
in hplc chromatogram started from left to right and in uv spectrum started from left to right
how to selecet an exact coloumn for an new molecule development by hplc how to select exact salt as buffer for new molecule development by hplc what is the the process to select the mode of saparation of compoundes by hplc what is the use of ph of buffer what is use of buffer,ph,organic phase,ans methods how the molecules get saparated in coloumn,
can i use hplc detector to uplc and why?