What is principle of Polarimeter?

Answer Posted / bharath

Polarimetry measures the rotation of polarized light as it
passes through an optically active fluid. The measured
rotation can be used to calculate the value of solution
concentrations; especially substances such as sugars,
peptides and volatile oils. A polarimeter consists of a
polarized light source, an analyzer, a graduated circle to
measure the rotation angle, and sample tubes.

The polarized light passes through the sample tube and
exhibits angular rotation to the left (-) or right (+). On
the side opposite the polarizer is the analyzer. Using
optics, visual fields are manually adjusted by the user to
measure the optical rotation angle.

Polarimeters offer high accuracies where precision is
critical in determining the concentration of samples. Cole-
Parmer offers manual polarimeters where you look through a
viewing scope to read values on a vernier scale, and
semiautomatic polarimeters that have a digital display.
Polarimeters can measure in angle of rotation (¡),
International Sugar Scale (°Z), or both.

Is This Answer Correct ?    19 Yes 2 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

IS THERE ANY EQUIPMENT TO CHECK AND CALCULATE THE POLARITY OF A LIQUID?

1985


HI,I CLEARED BOB CLERK EXAM. MY INTERVIEW WILL BE ON 9TH OCTOBER,2010.PLEASE SEND ME INTERVIEW QUESTIONS AND ANSWERS. THANK YOU.

1735


What is viod volume and peak purity in HPLC?

2340


what is definition of validation? which components are followed give detail?

1827


How can we calculate offset value pH measurment

1069






What is column in chromatography?

1761


Identify problem faced when mass spectroscopy is used with HPLC system ?

1380


What is importance of pH in the Heavy metal test and How it effect on the test results?

1754


What is the difference between purge septum flow and column flow in gas chromatography?

1837


What if impurity area in control sample coming more as compared to LOQ level of impurity ?

1146


what is the disso medium for tropsiun chloride

1798


What is a difference between potency and purity?

642


For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?

3493


What are usp limits for theoritical plats,resoution,tailing factor,peak to valley ratio

1341


what is impurity profile. how to interpret this impurity profile to a drug product or drug substance.

2200