Answer Posted / dinesh.m

use of the buffer is maintain the PH of the solution.........
Ex: Cyanodiole-HBr analysis in hplc v have use 3PH buffer solution as a mobile phase........

SUB:hard water means water having MgCl & CaCO3 r CaCl2
but v have use the ammonia buffer in titration with EDTA is complex formation.............
first we have add ammonia buffer to hard water MgCl2 is form the complex with NH3Cl as metal complex............After addition of EDTA. EDTA replaces the NH3 After completion of reaction the solution libarates EDT Acetate. This acetate react with E.Block-T give end point..............

USP methodology, EP methodology, IP methodology, among three if possible to use one methodology for qualify working standard to use USP, EP, IP ? Please explain...

1561

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what is mean by extactable and leachable study?

575

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is it necessary to do HPLC calibration at wavelength 315nm if we are doing analysis at this wavelangth

1670

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why xterra column require to use at higher ph?

436

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what is difference in ods I ods II is this is realy from sorbent / packing characteristics or from other means

1496

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How do we fix the sample concentaryion in hplc method development. What is the basis?

682

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What is the principle of Thermal conductivity detector and FID?

2261

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what are the standard methods for HPLC?

2250

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CAN WE DETERMINE THE PEAK PURITY IN GAS CHROMATOGRAPHY ANALYSIS?

711

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sop of a uv visible spectrophotometer double beam elico model

5357

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[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?

316

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A ha tc is actual defination of retention time?

1067

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How can I develope method of dissolution by HPLC OR UV

1893

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why require to add enzymes in capsules dissolution only? is it require to tablet also?

272

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Which is the highly polar and highly non polar column in HPLC?

1787

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