Generally for Normal Phase HPLC,stationary phase is Polar.In
place of polar if we take non-polar means what happened & why?
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Answer Posted / amol
In normal phase , stationary phase is polar while mobile
phase is non polar & separation is achieve by the polarity
of the compound. More polar compound eluted out first while
non polar compound eluted last . if we take non polar
stationary phase it will happen reverse..
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