Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
- 60 Answers
- 187671 Views
- Arvind, Aurobindo, Cipla, Reddy Labs, Shasun Chemicals, I also Faced
- E-Mail Answers
Answer Posted / devendrakumar
1. caffeine easily available in market.
2. In general we all use caffeine as a standard for
calibration of HPLC specially for (Calibration of Wave
Length Accuracy) since it will scan the whole of the UV
range i.e It gives a multiwavelength response like
first maxima at 205 nm
second maxima at 273 nm &
minima at 245nm
3. It is very stable, we can use a long time for analysis
i.e. it is not degradable
4. In this case we can use Erbium Perchlorate which will
scan the whole range(200 nm to 600 nm).It is stable and
little costly but not easily available in market.
d.devendrakumar
| Is This Answer Correct ? | 100 Yes | 5 No |
Post New Answer View All Answers
How to establish relative response factor for hplc. Why it is required?
how to qualify the impurity?
Basic principle of ESI?
Please tell me about the pH of Polycaboxylic ether is it in the 5-6 range ever or more than 6
What is related substance by HPLC impurity limits as per USP?
what are the guidelines for analytical method validations?
What is split ratio in Gc? Splitless? how requirr to select?
WHAT IS THE PURPOSE WE USE HCL, CHOPPER, BURNER, MONOCHROMATOR in AAS? PLEASE EXPLAIN BRIEFLY
Difference between hlaf and rlaf
What are the primary considerations in extractables and Leachables ?
WHAT IS THE USE OF STARTER ?????????
How do we fix the sample concentaryion in hplc method development?
can we use the same detector in HPLC as well GC and what could be the differences we can find in the final chromato graph in any aspects?
What is viod volume and peak purity in HPLC?
In Assy by potentio metry titration method how select MET U ,DET U, SET U,
