how do start hplc method development?
how much sample required for developing the method
development?
- 3 Answers
- 51116 Views
- BMS, Cipla, Indoco Remedies, Sun Pharma, I also Faced
- E-Mail Answers
Answer Posted / kizar ahamed
Method development starts with solubility of the
compound,if itis soluble in water v can try with reverse
phase if is is not v should use normal phase
chromatography.
2)selection of wavelength
3) column selection
4)Gradient setting(Always develope the method by using
gradient than isocratic)
| Is This Answer Correct ? | 71 Yes | 25 No |
Post New Answer View All Answers
how can give the expiry period and restadardisation of volumetric solution
In Assy by potentio metry titration method how select MET U ,DET U, SET U,
While performing TOC sst analysis Zero shift disabled & sample analysis zero shift enabled why?
Why use only methanol,used determinationof moisture content not use other solvents
Explain the relations between number of carbon atoms in alkanes and retention time ?
why salisylic acid not using now days for disdolution calibration?
what is partion and column chromatography
What is the difference between purge septum flow and column flow in gas chromatography?
What should be the level/ Percentage of Arsenic in Arsenic free zinc.
how decide the clining method and cleaning method validation require for this perticular products?
what is mean by 40 in the dissolution basket mesh size?
if tech transfer fails whay require to do?
Why potassium dichromate is used in calibration of uv?
in which situation require to change rs specification?
in which situation require to use paddle and basket?
