Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test

Answer Posted / atul ingle

Why we use it, Because of

# It gives a multiwavelength response like 205,245,273 nm
# It is easily available in market
# It is very stable, we can use a long time foranalysis
i.e. it is not degradable
# It is chip

Is This Answer Correct ?    173 Yes 13 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

why require to do water content for drug product?

319


how to get accurate result for Residue on evaporation in purified water

1124


how to calculate elemental impurities?

549


How can I develope method of dissolution by HPLC OR UV

1893


How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?

2717






Why irmoisture balance is used for corrugated box moisture testing

1929


Which is the highly polar and highly non polar column in HPLC?

1785


2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.

1764


why require the ph, buffer during hplc mobile phase?

407


Please give idea about method development for cleaning method and how maco level establish

275


In which situation we require to analytical method validation of excipient?

297




1819


what is impurity profile. how to interpret this impurity profile to a drug product or drug substance.

2204


For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?

3500


If vendor having more imp than monograph then how to proceed? and how to set spec?

340