Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
Answer Posted / satya prasad
it will detect at dual wave length and easily available in
market
| Is This Answer Correct ? | 253 Yes | 56 No |
Post New Answer View All Answers
in each media we require to use SLS? how to proceed?
What is viod volume and peak purity in HPLC?
how to calculate elemental impurities?
How we choose the mobile phase for method development.?
What is mean by PDR?
what is the structure of DOWEX-50
why sre you used Potassium hydrogen phthalate in standarisation of 1N NaOH and 0.1 N Perchloric Acid?
which are the diffrent batches in the pharmaceuticals?
Why we select scan range from higer wavelength to lower wavelength in uv visible spectroscopy and ftir spectroscopy ? .
What is importance of pH in the Heavy metal test and How it effect on the test results?
What is Band broading effect explain
can we use the same detector in HPLC as well GC and what could be the differences we can find in the final chromato graph in any aspects?
In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
What is the requirement for brazil in the analytical method vslidation comparr to ich?
why octanol used to determine the partition coefficient ?