Answer Posted / neha
Phenyl isothiocyanate reacts with free amino grp of
aminoacid or peptide under alkaline condition to form
phenyl isothiocarbomyl derivative or peptide which inturn
release water molecule in presence of Hcl to form
phenylthiohydantoin(PTH) & release remainder of peptides
for second reaction.
This reaction is also used to identify N-terminal
aminoacid in protein sequencing method
| Is This Answer Correct ? | 7 Yes | 1 No |
Post New Answer View All Answers
Define the phenomenon resonance?
Garnet is the ore of which element?
Which equation gives the relation between specific rate (k) and temperature?
What is meant by substrates of enzymatic reactions?
What are species grow in waitland?
Which metal is more metallic in nature among 1A group elements?
which fat soluble vitamin has co-enzymic function?
What is the name for magnesium per chlorate and what is its formula?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
How do peptides react with cyanogen bromide?
What is dipole?
What is the numeric relation between pyrimidine and purine bases in the dna molecule? Is that relation valid in rna molecules?
Bleaching powder is obtained when cl2 is passes through which inorganic compound?
Differentiate between gram +ve and gram -ve bacteria.
What are respectively some remarkable functions of cd4?
