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Answer Posted / neha
Phenyl isothiocyanate reacts with free amino grp of
aminoacid or peptide under alkaline condition to form
phenyl isothiocarbomyl derivative or peptide which inturn
release water molecule in presence of Hcl to form
phenylthiohydantoin(PTH) & release remainder of peptides
for second reaction.
This reaction is also used to identify N-terminal
aminoacid in protein sequencing method
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